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Foundation

FOUNDATION – Biochemistry

Compiled Topical Questions of Foundation – Biochemistry

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This connective tissue mucopolysaccharide builds up in Hunter’s disease, leading to organ damage and skeletal abnormalities.

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Which mucopolysaccharide accumulates due to the deficiency of iduronate sulfatase in Hunter’s disease?

Consider the directionality of DNA polymerase and the differences between the leading and lagging strands during DNA replication.

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Okazaki fragments are formed on the ______ strands in the ______ direction.

This lipid molecule stabilizes the membrane, so its reduction makes the membrane more fluid and flexible.

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Decrease in which of the following increases the fluidity of the cell membrane?

Think about what kinds of chemical bonds might be important for holding a protein’s 3D shape together. What special “ingredient” does IT have that the other amino acids lack?

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Which of these is a sulfur-containing amino acid?

“Think about the building blocks of a typical fat molecule like a triglyceride. What molecule connects the fatty acids together through ester bonds, forming the structure of fats and oils?”

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Lipids are formed by the condensation of which substances?

Consider the enzyme that breaks hydrogen bonds between DNA strands to make the template available — which one handles this mechanical task during processes like replication and sometimes transcription?

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During transcription of DNA which enzyme is responsible for unwinding the double helix?

“Which enzyme helps your body break down fats from your diet so they can be absorbed and used for energy?”

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Triglycerides are hydrolyzed by which of the following?

Think about the role of water in enzyme-catalyzed reactions. Which enzyme class does not rely on water to break chemical bonds but instead builds new molecules?

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Which enzyme does not belong to the class hydrolases?

Consider the plant-based foods rich in iron, especially those that are dark green and leafy. Iron from plants requires a little help with absorption (like vitamin C), but these foods are some of the best natural sources.

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Which of the following is the best source of iron?

Think about the tissue where transparency and hydration are critical for function, and the specific GAG that contributes to these properties.

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Which of the following structures contains keratin sulfate in abundance?

Think about the structure of RNA and how it can form secondary structures. In rho-independent termination, a specific sequence in the RNA transcript forms a stable structure that helps terminate transcription. What kind of sequence would allow for strong base pairing and a stable loop?

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In prokaryotes, the mechanism of rho independent termination of DNA replication involves which of the following?

Think about the bases involved in DNA vs RNA. Which base is unique to DNA and is absent in RNA, replaced by something else?

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Which of the following bases is not present in RNA?

Think of the amino acid with a cyclic side chain that doesn’t allow the polypeptide to maintain a regular helical shape. This causes a disruption in the usual helical structure.

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Which amino acid can disrupt helical structure?

Focus on the components of glycosphingolipids and the role of ceramide as an intermediate.

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Cerebrosides are composed of which of the following?

Not all enzymes are made of proteins—some can perform catalysis using a different type of biological molecule. Which fundamental biomolecule can store genetic information and also act as a catalyst?

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Which of the following is an enzyme made up of RNA?

“Consider which enzyme’s absence would prevent the removal of sialic acid residues from glycoproteins and glycolipids—leading to their accumulation in lysosomes. Which option hints at a connection to the very name of the disease itself?”

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In sialidosis, which of these enzymes is absent?

Think about the function of each structure during both rest and activity. Which of these would require control from the system that promotes relaxation, secretion, and conservation of energy? Consider how each system affects organs related to breathing, circulation, and glandular activity.

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Which is the only structure that receives a parasympathetic supply?

This vitamin is essential for NAD/NADP formation, and its deficiency leads to the classic “3 Ds”—skin rash, GI issues, and mental disturbances.

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A patient came to the outpatient department with a condition that causes a dark, sometimes scaly rash to develop on skin areas exposed to sunlight. He also complained of bright redness of the tongue and constipation/diarrhea. Which deficiency causes this condition?

Think about where hydrogen bonds form in a protein—are they between nearby amino acids or distant ones? How does this relate to the level of structure?”

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Beta-pleated sheat in an example of which of the following? Question’s Explaination and Hint is for a different question, please fix.

Think about the most basic level of protein structure. What term describes the linear order of amino acids in a polypeptide chain?

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What is the simple sequence of amino acid called?

Think of the “blueprint of life”— including the amino acids which form proteins.

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There are more than 300 amino acids in nature yet only 20 amino acids are found in humans for the purpose of protein formation. Which of the following is responsible for the involvement of only 20 amino acids for protein formation?

Focus on the buffer system that directly involves a gas that is actively regulated by the lungs. This system has a significant capacity to neutralize acids and is central to maintaining blood pH through both respiratory and renal mechanisms.

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Quantitatively, which of the following is the most significant buffer system?

Think about the classic base-pairing rule in DNA — always go back to that pairing when in doubt.

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In DNA, the two strands held together by base pairing follow Chargaff’s rule. According to this rule, which base does adenine pair with?

 

This phospholipid is the main component of surfactant, essential for reducing alveolar surface tension in newborns.

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The deficiency of which of the following lipids can result in respiratory distress syndrome in pre-term infants?

This small non-coding RNA molecule prevents gene expression by binding to mRNA and inhibiting its translation.

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Which of the following takes part in the regulation of gene expression?

Think about the fatty acids that the human body cannot produce on its own and must obtain from food. Which one is an omega-6 fatty acid commonly found in plant-based oils?

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Which of these is an essential fatty acid in the human diet?

Cholesterol acts as a fluidity buffer — think about where it needs to be to interact with substances and regulate membrane stability.

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Where are cholesterol moieties located in the cell membrane?

Think about the modifications that protect mRNA and help it interact with the translation machinery.

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What is added to the 5′-prime end of mRNA in eukaryotes?

What prevents freshly made mRNA from being “eaten up” by cellular enzymes that degrade nucleic acids?

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During post transcriptional modifications, a 5’ cap is added to the mRNA. What purpose does this serve?

 

Consider which molecule in the ETC is involved in transferring electrons in a one-electron fashion, particularly early in the chain. This molecule helps set up the next step in the chain by moving electrons one at a time.

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Which of the components of the electron transport chain accepts only 1 electron?

This energy storage form consists of three fatty acid chains attached to glycerol and is stored in adipose tissue.

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Fat is stored in the body in what form?

Remember that there is a start codon, which always brings in the first methionine — this sets the stage for the entire translation process.

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Which of the following is the base sequence for a start codon?

Think about the structure of the mitochondria and where the processes of the electron transport chain occur. Which part of the mitochondria is directly involved in creating the proton gradient necessary for ATP synthesis?

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In which of the following are four large complexes of the respiratory chain embedded?

Imagine trying to coil a tightly wound spring but inserting a stiff, non-bending segment in the middle. What would happen to the overall shape? Which amino acid has a structure that would force a bend in a tightly packed coil?

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Which of the following amino acids breaks the alpha-helix winding?

“Which lipid-derived molecules are involved in inflammation, blood clotting, and allergic reactions?”

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Which is the signaling molecule made from the oxidation of arachidonic acid or other polyunsaturated fats?

Consider the direction in which RNA primers are removed and think about what type of enzymatic activity allows cutting nucleotides starting from the end where synthesis began. What exonuclease direction would make sense for moving along and clearing out primers?

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DNA polymerase 1 is able to remove an RNA primer due to which activity?

This anticoagulant glycosaminoglycan contains a highly sulfated sugar with a unique uronic acid that enhances antithrombin activity.

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Which repeating disaccharide units make the anticoagulant polymer heparin?

Think about the level of protein structure that involves the overall 3D shape of a single polypeptide chain, stabilized by interactions between the side chains of amino acids. Which level of structure does this describe?

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3D folding of protein due to side-chain interaction is classified as which structure of a protein?

Consider the metabolic pathways and physiological conditions that might increase the demand for certain amino acids. Which amino acid is needed by the body during periods of growth or stress?

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Which of these is a semi-essential amino acid?

“Which ECM glycoprotein exists in both a structural form (in tissues) and a soluble form (in plasma)?”

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Which is the major glycoprotein of the extracellular matrix that is also soluble in plasma?

Think about which amino acid’s metabolic pathway branches into NAD+ synthesis—and why diets poor in this amino acid (but seemingly adequate in niacin) can still cause deficiency symptoms.

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Which of the following amino acids is used to synthesize niacin?

This amino acid is an essential amino acid, playing a role in methylation reactions.

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Which of the following is a sulfur-containing amino acid?

Consider which coenzyme is directly involved in redox reactions and is closely associated with the flavin group of molecules. This coenzyme is derived from riboflavin, a vitamin essential for various metabolic processes.

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Which coenzyme is derived from the vitamin riboflavin?

Consider what influences whether a fatty acid is solid or liquid at room temperature.

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What do the properties of fatty acids depend on?

Think of the fatty acids we must eat because our body lacks the enzymes to make them. These play a key role in cell membrane structureinflammation control, and brain function

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Which of these is our body unable to synthesize?

Think about the physical stress that occurs when the DNA double helix unwinds during replication. What enzyme would be needed to prevent the DNA from becoming overwound and tangled?

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Why is topoisomerase required in DNA replication?

Think about the molecular structure of water and how its partial charges interact with ions. What property of water allows it to surround and separate ions in a salt crystal?

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Why can water dissolve large quantities of salts?

LDL plays a key role in lipid transport and is often linked to cardiovascular health. Which characteristic makes it significant in this context?

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Which of the following is correct regarding LDL?

Think about what the “R” in RNA stands for — it hints directly at the sugar involved.

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Which of the following is the sugar involved in RNA?

 

Consider the glycoproteins that play a role in both tissue structure and blood-related processes. Which one bridges the extracellular matrix and the circulatory system?

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Which is the major glycoprotein of the extracellular matrix that is also soluble in plasma?

The number of bases in a codon determines how many different amino acids can be coded for. Consider the number of unique nucleotide bases and how they combine to form meaningful sequences.

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Genetic code is responsible for coding for an amino acid in proteins. The genetic code consists of how many bases?

 

Arachidonic acid belongs to the omega-6 family of fatty acids. Which essential fatty acid serves as its precursor and must be obtained from the diet when it is absent?

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The deficiency of which one of the following fatty acids in diet makes arachidonic acid an essential amino acid?

Think about which lipid type provides the most concentrated energy source and is stored in adipose (fat) tissue — it’s a combination of glycerol and fatty acids.

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Which of the following is the main form of lipid storage in the body?

Think about the modifications that protect mRNA and help it survive longer in the cell. What structure is added to the “tail end” of the mRNA molecule?

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During the posttranscriptional modification of mRNA which of the following is added at the 3′ end?

Think about the final step in DNA replication or repair—what enzyme is essential to stitch together the backbone after other enzymes have synthesized or removed damaged sections?”

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What is the function of DNA ligase?

Look for the amino acid that has a benzene ring as part of its side chain. This type of structure is key to identifying aromatic amino acids.

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Which of the following amino acids has an aromatic side chain?

think about how oil behaves in water. Since fatty acids are a major component of oils and fats, would they mix with water or repel it?

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The non-polar nature of fatty acids makes them show which of the following characteristics?

Consider what molecule provides immediate energy for most active cellular processes!

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In cellular transport, the Na-K pump is a critical transport mechanism that uses energy. The energy required for this transport is directly obtained from which of the following?

Think about the amino acid that’s the “starting point” for stress hormones and thyroid function. What’s the common precursor that ties these powerful molecules together?

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The hormones epinephrine, norepinephrine, and thyroxine are derivatives of which of the following amino acids?

Think of an enzyme like a lock, and the substrate as a key. The key fits into a specific part of the lock where the reaction happens—what is that part called?

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At what site, within an enzyme, does the catalytic action of an enzyme generally take place?

This monounsaturated fatty acid (ω-9) is commonly found in cell membranes and olive oil, contributing to membrane fluidity.

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Which of the following is the most abundant unsaturated fatty acid in membrane lipids?

An acid is half-ionized when the concentration of its protonated and deprotonated forms are equal — this balance defines a key point in its dissociation curve.

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In which of the following is an acid half-ionized?

“If an inhibitor can be outcompeted by simply increasing substrate concentration, it must be fighting for the same spot as the substrate. Which type of inhibition does that sound like?”

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In which type of inhibition do a substance bind to the active site and the substrate compete with that substance to occupy the active site?

Think about the branched component of starch that allows for rapid energy release due to its multiple ends for enzymatic digestion.

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Which type of starch has both α 1-4 linkages and α 1-6 linkages (at branch points)?

Think about whether the body can produce tyrosine on its own or if it must be obtained through the diet. Consider the relationship between tyrosine and phenylalanine, another amino acid.

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Tyrosine falls under which class of amino acids?

“Which polysaccharide contains glucose units linked by β-1,4 linkages, providing rigidity and resistance to digestion?”

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Which of the following has a beta 1,4 glycosidic linkage?

Consider the molecule that efficiently packs the highest energy density while remaining chemically inert in storage depots. What structural features allow it to hold the most calories per gram without interfering with cellular functions.

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Fat is stored in the body in what form?

Think of the 3′ end of tRNA as the “loading dock” for amino acids…

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Which of the following is present at the 3′ end of a tRNA?

This factor acts like a “navigator” — without it, the RNA polymerase wouldn’t know where to start transcription. Once the right spot is foundit steps aside, and the main enzyme takes over.

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In prokaryotes, initiation of the transcription involves recognition of the initial site by which factor of RNA polymerase enzyme?

Think about what needs to happen to the DNA double helix before it can be copied. What enzyme in DNA replication breaks bonds between paired bases?

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Which of the following enzymes is responsible for the separation of two strands of DNA?

Consider which hormone is essential for allowing cells to take up glucose and preventing the breakdown of fats into acids under normal conditions. What happens when that hormone is absent?

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What is deficient in diabetes ketoacidosis?

Think about the structure of fats and oils. What three-carbon molecule serves as the backbone for these energy-storage molecules, allowing them to bind three fatty acids?

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Triacylglycerols are esters of fatty acid with which of these?

“Which level of protein structure involves local folding patterns such as alpha helices and beta-pleated sheets?”

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Beta-pleated sheat in an example of which of the following?

“What type of molecule stores the most energy per gram in the human body — and what kind of chemical bonds help it do that?”

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Triglycerides are formed by which of the following molecules?

Imagine two nearly identical keys that open the same lock, but one has a single tooth shaped differently. This minor structural change does not make them opposites, nor does it alter their fundamental function—yet it distinguishes them from each other. In molecular terms, what type of relationship does this represent?

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Why are glucose and mannose epimers?

Think about the chemical structure of glycosaminoglycans. What type of sugar derivative is commonly found alongside amino sugars in these complex carbohydrates? Focus on the functional groups that contribute to the unique properties of GAGs.

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Which of the following, along with amino sugars, constitutes the complex carbohydrates called glycosaminoglycans?

These enzymes move functional groups like phosphates, methyl groups, and amino groups from one molecule to another.

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Which type of enzymes transfers functional groups?

This polysaccharide provides rigidity to plant cells and is indigestible by humans, making it an essential part of insoluble fiber.

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Which polymer found in plant cells is a major component of their cell and is also a major constituent of insoluble dietary fiber?

These proteins are not embedded in the membrane but attach temporarily, often regulating important metabolic actions.

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What is the function of peripheral proteins in the cell membrane?

 

Think about the relationship between the inhibitor and the substrate. If the inhibitor and substrate are “fighting” for the same spot on the enzyme, what type of inhibition would this represent? How does increasing the substrate concentration affect this competition?

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In which type of inhibition do a substance bind to the active site and the substrate compete with that substance to occupy the active site?

Think about the definition of optical isomers. What property distinguishes them from other types of isomers, and how does this relate to their interaction with light?

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Which of the following interactions are responsible for high viscosity, high surface tension, and high boiling point of water?

Think about the structural polysaccharide in plant cell walls that humans cannot digest but is essential for dietary fiber.

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Which polymer found in plant cells is a major component of their cell and is also a major constituent of insoluble dietary fiber?

What word would refer to the number 7?

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Which sugar has 7 carbons?

“Imagine a crowded subway car. If everyone stands stiff and straight, movement is restricted. But if some people bend their arms and shift their posture, the space becomes more flexible. Which molecule contributes to this state?”

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Which of the following contributes to the fluidity of the cell membrane?

Think about enzymes that perform the same function but are adapted to work in different tissues or conditions. These enzymes are commonly used in medical diagnostics (e.g., CK-MB in myocardial infarction).

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Which of the following is correct for isoenzymes?

Think about which part of RNA polymerase is only temporarily associated during the start of transcription to help locate the promoter — and then leaves once transcription begins.

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In prokaryotes, initiation of transcription involves recognition of the initial site by which factor of RNA polymerase?

“Think about the cell type in the nervous system that relies heavily on membrane structures for signal transmission, where complex lipids like gangliosides play a crucial role in cell communication, recognition, and neurodevelopment.”

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Gangliosides are complex sphingolipids mostly found in which of the following cells of the body?

Think about the role of different ribosomal sites during translation. Where does the first tRNA bind when translation starts, and how does this position influence the growing polypeptide chain?

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During translation, the initiation codon of mRNA attaches to the ribosome at which site?

 

These amphipathic molecules, secreted by the liver and stored in the gallbladder, help break down fat globules into micelles for easier digestion by enzymes.

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Emulsification of fats is a process in which large complex fat globules get converted into smaller fat molecules. Which emulsifying agent is present in the gastrointestinal tract for the digestion of fats?

“Where does the substrate bind for the reaction to take place?”

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At what site, within an enzyme, does the catalytic action of an enzyme generally take place?

Think about a glycoprotein that bridges the gap between cells and the ECM while also circulating in the blood to assist in tissue repair and clotting. Which protein fits this description?

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Which is the major glycoprotein of the extracellular matrix that is also soluble in plasma?

Think about the small RNA molecules that can inhibit or decrease the expression of genes without altering the DNA sequence itself. Theyre like silencers of gene activity.

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Which of the following takes part in the regulation of gene expression?

This condition is characterized by bowed legs in children due to defective bone mineralization caused by low calcium and phosphate levels.

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Which of the following is caused by the deficiency of vitamin D?

“Which organelle contains genetic material, controls gene expression, and plays a key role in cell division?” It’s the control center of the cell

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Which of the following cell organelles has lamins (proteins) in their membrane, consists of DNA and histone, has chromatin, and controls cell division?

Think about the relationship between pKa and the tendency of an acid to donate protons and proportionality of pKa with strength of an acid.

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A high pKa value refers to which of the following?

Imagine you have a bunch of pipe cleaners and you can bend and connect them. Could you make different shapes that all use the same number of pipe cleaners? Now, if you could also twist those shapes in 3D space, that’s where you start to get into optical isomers.

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What is the class of isomers that differ in structure because of the different arrangement of functional groups around the carbon atoms called?

These strong covalent bonds are responsible for stabilizing multi-subunit proteins and can be broken by reducing agents.

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In quaternary structure, cysteine residues interact with the other polypeptide chains with the help of which of the following bonds?

Think about the type of mutation that changes a single nucleotide in a way that alters the amino acid sequence without disrupting the reading frame or introducing a stop codon.

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Which type of mutation leads to the addition of a different amino acid in the sequence?

Consider the role of different enzymes in DNA replication. Some are responsible for making new strands, while others assist by modifying or preparing the DNA. Which enzymes need to ensure accuracy at the nucleotide level, and which ones can function without directly interacting with nucleotides?

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Which of the following does not have any proof-reading activity?

 

Consider the role of the ribosomal components in translation. Which part of the ribosome first interacts with the mRNA to ensure proper alignment before protein synthesis begins?

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The first step in protein synthesis is mRNA binding to which of the following?

🔍 Think about the waterproof substances found in nature. These lipids are made from a fatty acid and a long-chain alcohol.

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Which of the following substances is formed due to the esterification of fatty acids with long-chain alcohols?

Think about the structure of ATP and the negative charges on its phosphate groups. Which ion is known to interact with these charges to stabilize ATP and make it a better substrate for enzymes?

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Enzymes that use ATP as a substrate or cofactor require which of the following?

Look for the compound that is a lipid with carbohydrate groups attached, and is known to play a role in processes like cell signaling, which can be affected during abnormal cell growth.

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Carbohydrate changes in dysregulated cell growth in lipid molecules are characteristic of which type of compound?

Think about the lipoprotein that’s the “delivery truck” for dietary fats. Which one is packed with the most triacylglycerol?

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Which of the following has the highest concentration of triacylglycerol (TAG)?

Consider the process where fatty acid chains are attached to a backbone molecule like glycerol. What is this backbone made of, and how does the condensation process work?

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Lipids are formed by the condensation of which substances?

Consider the role of vitamins in enzymatic reactions. Which substance is known for its involvement in maintaining the structural integrity of connective tissues and is historically linked to a disease caused by its deficiency, characterized by weakened collagen?

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During collagen synthesis, what does the formation of hydroxyproline residues require?

Buffers are designed to maintain pH stability by neutralizing both acids and bases. Consider which pair of substances has the ability to do this most effectively by interacting with H⁺ ions.

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What is a buffer system composed of?

Think about the types of modifications that do not involve the formation or breaking of covalent bonds. Which process involves the addition of carbohydrate groups to proteins?

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Noncovalent modification after translation includes which of the following?

“Why does olive oil stay liquid on your kitchen counter while butter stays solid? Think about the chemical structure and how it affects physical properties.”

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At room temperature, unsaturated triglycerides are found in which form?

“DNA polymerase has a one-way road—how does it handle a strand running in the opposite direction?”

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Okazaki fragments are formed on the ______ strands in the ______ direction.

Think about the two factors that determine whether a fatty acid is solid or liquid at room temperature. What’s the combination that defines its behavior?

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What do the properties of fatty acids depend on?

Focus and recall the definition of the molecule and their relationship to chirality and mirror-image configurations.

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Monosaccharides that are mirror images of each other are known as?

Think about which of these proteins has a distinct color due to a tightly bound non-protein component.

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Which of the following is an example of chromoprotein?

Think about the types of RNA that require structural stability and functional efficiency. Which RNAs are known to contain modified nucleotides like pseudo uracil to support these roles?

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Which type of RNA contains pseudo uracil?

This process converts DNA information into an RNA message, which is later used for protein synthesis.

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Which of the following describes the process by which mRNA synthesis is initiated, elongated, and terminated?

Think about an enzyme that can fill in missing nucleotides during base excision repair—not just unwind or seal DNA!

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Which enzyme has the ability to repair DNA?

Consider which chromosomal alteration involves an unequal exchange of genetic material between chromosomes, leading to missing or extra genes. How does this compare to other structural changes that primarily rearrange or duplicate genetic material without significant loss?

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A significant loss of genetic material is observed in which of the following chromosomal structure alteration?

“What is the first level of protein organization that dictates all other levels?”

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What does the primary structure of protein refer to?

Think about the structure of the DNA molecule after replication. How are the parental and newly synthesized strands distributed in the daughter molecules?

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Which of these is true for semiconservative replication?

Think about the term that describes molecules with the same “ingredients” but different “recipes.” What’s the general term for these variations?

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Molecules that have the same molecular formula but different structures are known as which of the following?

“Think of a sponge in cartilage—this molecule traps water, making tissues resilient and hydrated. What is it attached to?”

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Proteoglycans are proteins that are covalently bonded to which of the following molecules?

Consider the role of water dissociation in determining the concentration of hydrogen ions in a solution.

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Dissociation of water is an important property for which of the following?

Think about whether your body would want to keep the urine more acidic or more basic to help eliminate metabolic wastes and maintain overall acid-base balance. Where in that balance would a healthy individual typically be?

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What is the normal urinary pH?

This compound is orange-colored and is commonly found in carrots and sweet potatoes. The body converts it into Vitamin A.

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In which form is provitamin A found in vegetables?

These biomolecules are the primary source of energy in living organisms.

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Which of the following biomolecules contain a polyhydroxy aldehyde or a polyhydroxy ketone in its structure?

The first tRNA must bind where the polypeptide chain starts growing—which site does this in the ribosome?

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During translation, the initiation codon of mRNA attaches to the ribosome at which site?

Think about the group of vitamins that are the “helpers” in metabolic reactions. Which ones are directly involved in enzyme function?

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Which of the following vitamins acts as a coenzyme?

Think about the most abundant plasma protein that prevents water loss from blood vessels and carries hormones and drugs in circulation.

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Which is the major plasma protein that helps to transport substances and maintains osmotic pressure?

Think about the number of sugar units in maltose. If it’s made up of two glucose molecules, what category of carbohydrates does it belong to?

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Maltose is classified as which of the following?

Think of the phase where the “synthesis” of new DNA strands takes place, right between cell growth and preparation for division.

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DNA is replicated during which phase?

 

Think about the metabolic pathways where riboflavin plays a role—particularly those involving high-energy electron transfers. What molecules directly incorporate riboflavin to facilitate these reactions?”

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Riboflavin produces which coenzyme? – 

Think about the structure of chromatin and the role of the proteins. What biomolecule is tightly wrapped around histone proteins to form chromosomes?

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A chromosome has a protein conjugated with which of the following biomolecules?

“Think about the special modification added immediately after transcription starts, protecting the mRNA from degradation and helping it dock at the ribosome for translation initiation.”

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The mRNA transcript is modified at the 5′ end which aids it in binding to a ribosome and adds to its stability. What is this modification?

“If an acid gives up protons easily, does it hold onto them strongly or weakly?”

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Which of the following is true for strong acids?

“Which vitamin helps strengthen the tissues that hold your body together?”

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What does vitamin C deficiency lead to?

Consider how a cell might regulate its own activity without relying on external signals. What mechanism would allow a cell to ‘self-stimulate’ in response to its own secreted molecules?

 

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What is the type of signaling in which the signaling factors produce an effect on the cell from which they are produced called? –

Think about the midline structure in the embryo that’s important for skeletal development and lies just beneath the future spinal cord.

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Nucleus pulposus is a remnant of which of the following structures?

Think about the biochemical reactions in which vitamin B12 participates, such as the conversion of homocysteine to methionine. What is the name of the active form of vitamin B12 that is directly involved in this reaction?

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What is the activated form of vitamin B12?

“Think about the type of fatty acids that must come from your diet because your body lacks the enzymes to introduce double bonds beyond certain points in the carbon chain.”

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Which of these is our body unable to synthesize?

Think about the membrane where the proton gradient is established to drive ATP synthesis. Which mitochondrial membrane contains the protein complexes of the electron transport chain?

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Enzymes involved in the electron transport chain are located in which of the following?

Think about the “molecular mimicry” strategy—what type of inhibition involves an inhibitor that looks like the substrate and binds to the same spot?

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The structure of the inhibitor is similar to the structure of the substrate in which of the following types of enzyme inhibition?

This essential amino acid is a precursor for serotonin and melatonin, and it feeds into both glucose and ketone body production.

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Which amino acid has both glucogenic and ketogenic metabolic properties?

“Focus on the term ‘aliphatic’ — meaning straight or branched chain, not aromatic. Then think about which option contains only a carboxylic acid group without any additional functional groups like amines or hydroxyls.”

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Which of the following is an aliphatic compound with only a carboxylic acid group?

Focus on the classification of amino acids as essential or non-essential. Remember that essential amino acids cannot be synthesized by the body.

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Which of the following amino acids is not produced in the body and is therefore needed in our diet?

Among amino acids, think about which one has a unique structure that connects back to its backbone and would prevent the usual hydrogen bonds needed to maintain a helical structure.

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Which of the following amino acids can break down an alpha-helix chain?

This amino acid is synthesized from phenylalanine and is important in neurotransmitter and thyroid hormone production.

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Tyrosine falls under which class of amino acids?

Think about the enzyme that is responsible for ensuring the continuity of the DNA strand. Consider its role in DNA replication and repair.

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What is the function of DNA ligase?

Think about what happens to your food in your gut. Which of these carbohydrates might create a “sticky” environment that could trap fats? Which ones just get broken down and absorbed quickly?

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Which of the following carbohydrates modifies lipid absorption from the intestine?

Consider which highly reactive oxygen species, known for damaging DNA and lipids, can form when hydrogen peroxide interacts with metal ions like iron in biological systems.

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Tags: 2018

H₂O₂ can be hydrolyzed to produce which free radical?

Think about the bases involved in DNA vs RNA. Which base is unique to DNA and is absent in RNA, replaced by something else?

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Which of the following bases is not present in RNA?

“Which fatty acid is released from cell membranes and converted by COX enzymes into inflammatory mediators?”

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Which of the following fatty acids is the precursor of the prostaglandins?

“Which vitamin, essential for calcium homeostasis and bone health, is produced in the skin in response to sunlight and stored in fat tissues?”

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Tags: 2016

Which of these is a fat-soluble vitamin?

Think about the protein that provides tensile strength and is the most abundant in connective tissues. What is the primary structural protein in the body?

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What is the major component of most connective tissues?

Think about what part of a gene sequence would need to be “blocked” to prevent transcription from starting — where would a molecule physically sit to stop RNA polymerase from doing its job?

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Tags: 2018

In the lac operon, at which site is the repressor protein attached?

“Think about the primary carbohydrate stored in plants and animals for energy. Would it be built from a sugar that forms rigid fibers or one that allows quick energy release?”

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Tags: 2019

Starch and glycogen are polymers of which isomer of glucose?

Think about the structure of the amino acids. Which one has a “kink” built into its side chain? Which one would be least flexible and therefore most likely to disrupt a nice, neat helix?

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Which amino acid can disrupt helical structure?

Think of the orange and green leafy vegetables that are rich in this plant-based precursor of vitamin A, responsible for eye health and immunity.

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In which form is provitamin A found in vegetables?

Consider the key structural protein that contributes to the elasticity and strength of connective tissue. This protein is critical for maintaining the integrity of structures like the aorta and the eye’s supporting fibers. Identify the gene responsible for producing this protein, which is mutated in Marfan syndrome.

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A 20-year-old male presents to the outpatient department with a complaint of loss of vision in the right eye. He further mentions that his brother and cousin also had the same symptoms and were diagnosed with Marfan syndrome. What is the gene defect in this syndrome?

Think of the first biomarker to dash onto the scene like an emergency responder—but one that’s a bit too eager and not very specific.

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Tags: 2019

Which of the following enzymes is first raised in myocardial infarction?

In sickle cell anemia, a change from one amino acid to another causes a structural change in hemoglobin. Which amino acid is substituted for glutamic acid in the hemoglobin mutation?

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Tags: 2018

In sickle cell anemia, the mRNA codon is GTG. Which amino acid does this code for?

Consider what kind of complex sugar would be needed to form structural components like cartilage or the extracellular matrix — would it be made of only one type of sugar or a combination of different sugars?

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Which of the following is an example of mucopolysaccharides?

The ability to participate in redox reactions depends on having a reactive functional group. Think about the smallest units of carbohydrates that still retain this property.

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What are the sugars that either have a free aldehyde or ketone group?

Think about what part of the amino acid structure is different in each type of amino acid and directly determines its behavior and function in a protein.

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Tags: 2018

What do the characteristics of amino acids mainly depend on?

Think about the structural polysaccharide in plant cell walls that humans cannot digest but is essential for dietary fiber.

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Which polymer found in plant cells is a major component of their cell and is also a major constituent of insoluble dietary fiber?

“Think about what pKa really represents—it reflects how easily an acid donates a proton. If an acid is weak and holds onto its proton tightly, what does that say about its pKa value?”

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Tags: 2017

Which property do weak acids possess?

Think about the structure of unsaturated fatty acids. The bends caused by double bonds reduce their ability to pack together tightly. How might this affect their physical state when they are not packed closely?

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At room temperature, what state do unsaturated fatty acids exist in?

Focus on the mechanism by which ACE inhibitors reduce enzyme activity and how this affects Vmax and Km.

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A hypertensive patient is given an angiotensin-converting enzyme (ACE) inhibitor that works by lowering Vmax. What type of inhibition is most likely to be involved in this case?

Think about how digestive enzymes like trypsin, pepsin, and thrombin are activated from their inactive precursors. What kind of chemical change makes them functional?

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Tags: 2019

How is the zymogen enzyme activated?

Which codons signal the end of translation, not the addition of amino acids?

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Which of the following is a stop codon?

Consider what full building block is needed to make up a strand of DNA or RNA, and think about the components involved in linking these units together.

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A nucleotide is composed of which of the following?

 

These two hexoses differ only at the 4th carbon, and one of them is a major component of lactose (milk sugar).

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Epimers are carbohydrates that vary in one position for the placement of the OH group. Which of the following is an example of an epimer?

Think about the RNA molecule that is synthetically designed to bind to and degrade specific mRNA sequences, effectively silencing the expression of a target gene.

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Which RNA can stop the expression of the gene when introduced synthetically?

“Which type of acid dissociates more readily in solution and has a lower pKa?”

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What does low pKa refer to?

When you see molecules with identical formulas but different shapes or connectivity, think of the word that captures these structural variations!

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Molecules that have the same molecular formula but different structures are known as which of the following?

Think about the amino acid that acts as a “safe container” for ammonia in the body. What’s the end product of ammonia detoxification?

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Ammonia is converted into which of the following non-toxic amino acids?

When two sugars or carbohydrates are almost identical, but their spatial difference exists at just one carbon, that’s the hallmark of this type of stereoisomer.

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Carbohydrates that differ in configuration around a single carbon atom are known as which of the following?

Think about the metabolic pathway of fatty acids. Which essential fatty acid is the precursor for arachidonic acid, and what happens if it is not available in the diet?

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Tags: 2020

Arachidonic acid becomes essential when which of the following is deficient in diet?

Why does water boil at a much higher temperature than other small molecules like methane (CH₄) or ammonia (NH₃)?

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Tags: 2018

Surface tension, high viscosity, and high boiling point of water is due to which of the following?

This technique utilizes antibodies to identify specific molecules within a sample, after they’ve been separated by size.

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Which technique is used to detect proteins?

“Think about the very first step of Complex I. NADH arrives with two electrons—but iron-sulfur clusters, the next stop, can only accept one. Who’s the ‘middleman’ in this redox relay?”

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Tags: 2018

Which of the components of the electron transport chain accepts only 1 electron?

Think about the steps involved in gene expression. Which process occurs at the RNA level and is not related to protein modification?

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Which of these is not included in posttranslational modification of proteins?

Consider the nature of the reactions catalyzed by each enzyme class. Think about how the enzyme interacts with its substrate and what changes occur in the substrate as a result. Which class of enzymes is specifically designed to move functional groups from one molecule to another?

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Tags: 2020

Which type of enzymes transfers functional groups?

If mRNA must be complementary to one of the DNA strands, which strand would remain unchanged while still holding the same genetic information?

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Which DNA strand is converted into mRNA?

“Why do we wear sunscreen to prevent certain types of skin cancers — and what kind of DNA damage does it help avoid?”

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Tags: 2016

What can cause pyrimidine dimers in DNA?

Think about the Henderson-Hasselbalch equation. Which two components of a buffer system are directly involved in determining its pH?

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Tags: 2020

pH of buffer mainly depends on which of these?

Think of the enzyme responsible for forming strong covalent bonds between collagen and elastin fibers — and the metal ion cofactor it need

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Tags: 2019

Deficiency of which of the following causes defects in cross-linking of collagen and elastin fibers?

Think about the “energy savings account” of animals—what carbohydrate is stored in the liver and muscles for quick access?

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Tags: 2022


 

Which of the following carbohydrates serves as a stored form of energy in animals?

 

Think about what class of molecules helps hold water in tissues and gives the skin and joints their resilience.

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Tags: 2018

Hyaluronic acid can be injected to prevent wrinkles in the skin. Which of the following is hyaluronic acid classified as?

Think of the triplet of mRNA bases that directly determines the amino acid added to a growing protein chain — that’s your answer!

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Tags: 2018

What is the set of three bases in an mRNA that codes for a specific amino acid called?

 

Think about external physical factors that can directly cause DNA damage and mutations. One common source is the sun, which is known to cause genetic changes through its effects on DNA bases.

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Tags: 2021


Which process is involved in the DNA damage by mutation?

Think about how amino acids can have both acidic and basic groups. Is there a sweet spot where these charges balance each other out? What would you call that point?

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Tags: 2022

Which of the following terms is described as the point at which the net charge on an amino acid is neutral?

Think about the definition of optical isomers. What property distinguishes them from other types of isomers, and how does this relate to their interaction with light?

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Tags: 2020

Which of the following is incorrect regarding optical isomers?

Think about the specific enzyme that modifies proline residues in collagen to form hydroxyproline. What is the name of the enzyme that adds a hydroxyl group to proline?

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Tags: 2020

Which of these enzymes is used for the formation of hydroxyproline?

Think about the molecules involved in inflammation and immune responses. Which group of signaling molecules is synthesized from the oxidation of polyunsaturated fatty acids like arachidonic acid?

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Tags: 2020

Which is the signaling molecule made from the oxidation of arachidonic acid or other polyunsaturated fats?

Think about substances that are used for protective coatings. What chemical structure might they have?

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Tags: 2019

Which of the following substances is formed due to the esterification of fatty acids with long-chain alcohols?

“Which mineral is required for an enzyme that stabilizes collagen and elastin by forming cross-links?”

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Tags: 2019

The deficiency of which of the following minerals is seen in Menkes disease, an X-linked recessive disorder, involving the defective cross-linking of collagen and elastic fibers?

These long, negatively charged polysaccharides are essential for hydration and support in cartilage and connective tissues.

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Tags: 2023

Proteoglycans are conjugated proteins that contain carbohydrates in which form?

Think about which activity allows the enzyme to remove nucleotides in the direction of DNA synthesis.

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Tags: 2016

DNA polymerase 1 is able to remove an RNA primer due to which activity?

DNA needs help managing tension when it’s unwound for replication. Which enzyme is responsible for relieving this strain by introducing twists in the opposite direction?

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Tags: 2016

Which of the following produces negative supercoils in the DNA strand?

“Think of the something that helps remove non-coding sequences and make mRNA ready for translation — it works closely with protein partners in a complex called the spliceosome.”

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Tags: 2016

Which type of RNA is involved in splicing?

Think about the initiation phase of transcription in prokaryotes. What component of the RNA polymerase holoenzyme is responsible for recognizing the promoter and ensuring accurate binding to the DNA?

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Tags: 2020

Which is the component of holoenzyme that is used in transcription?

Imagine you have a molecule with several “knobs” that can be turned. If you only turn one knob, what kind of isomer do you create?

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Tags: 2021

Carbohydrates that differ in configuration around a single carbon atom are known as which of the following?

Think about what happens between transcription and translation to make mRNA functional — removing unnecessary sequences and connecting coding regions.

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Tags: 2018

The process involving removal of introns and joining of exons in mRNA is known as which of the following?

 

This condition involves rearranging genetic material within a chromosome.

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Which of the following is not classified as a structural chromosomal abnormality?

Think about the relationship between a raw material and the final product. Beta carotene is not the active form of vitamin A but is transformed into it in the body. What term describes a compound that serves as a precursor to an active vitamin?

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Tags: 2020

What is the function of beta carotene in relation to vitamin A?

Think of the vitamin deficiency disease that leads to weak collagenfragile blood vessels, and impaired wound healing

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Tags: 2019

Which cofactor is required for the hydroxylation of proline?

Think of the courier of the celldelivering functional groups from one molecule to another!

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Tags: 2019

Which enzyme causes the transfer of functional groups from one molecule to another?

Consider which enzyme directly manages the tension created in the DNA helix during unwinding processes. This enzyme works by temporarily breaking the DNA strands to allow the molecule to relax before resealing them.

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Tags: 2021

Which enzyme is responsible for releasing DNA supercoils?

Think about the fatty acids that your body can’t make on its own—what must you get from your diet? Focus on the omega-3 and omega-6 families.

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Which of the following are essential fatty acids?

Think of the nucleus as a high-security vault. What’s the “file organizer” that keeps DNA tightly packed yet accessible for action? It’s not just a storage unit—it’s the master regulator of genetic expression. What’s this DNA-protein complex called?

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Tags: 2022

Which of the following parts of the nucleus is composed of histone proteins and DNA?

This RNA molecule does not encode proteins but instead modifies mRNA function by silencing genes.

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Which of the following takes part in the regulation of gene expression?

As DNA unwinds, it twists and tangles—which enzyme cuts and relaxes it to prevent damage?

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Which enzyme is responsible for releasing DNA supercoils?

Think about the physical state of vegetable oils (unsaturated fats) compared to butter (saturated fats) at room temperature. What does this tell you about the melting points of unsaturated fatty acids?

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Tags: 2020

Which of the following is true for unsaturated fatty acids?

This secondary structure is a right-handed coil, found abundantly in fibrous and globular proteins, and is stabilized by hydrogen bonding between every fourth amino acid.

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Which secondary structure is characterized by a single, spiral chain of amino acids and is stabilized by hydrogen bonds?

Consider what kind of bond is weak enough to allow DNA strands to separate during replication, yet strong enough to keep them paired when needed.

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Tags: 2018

The two DNA strands are held together in a helix by certain bonds. Which of the following best describes these bonds?

 

This polysaccharide makes up the branched portion of starch and allows for rapid breakdown by enzymes like amylase.

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Tags: 2021

Which type of starch has both α 1-4 linkages and α 1-6 linkages (at branch points)?

The immune system uses molecular markers to differentiate self from foreign invaders. What kind of molecules on the cell membrane might serve as “identification tags” for this purpose?

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Tags: 2019

Which of the following is incorrect regarding carbohydrates?

Think about the amino acid that is metabolized through a pathway involving the production of NAD+ (nicotinamide adenine dinucleotide), a derivative of niacin.

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Tags: 2020

Which of the following amino acids is used to synthesize niacin?

Once inside the nucleus, what process turns DNA instructions into a message that can be used to make proteins? Think of the step that comes before translation!

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Tags: 2018

Thyroid hormone enters the cell and interacts with receptors present inside the cell. The complex of hormones and receptors enter the nucleus and initiate the making of RNA from DNA. What is this process called?

 

Which small RNA is commonly used in laboratory experiments and gene therapy to silence specific genes by degrading mRNA?

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Tags: 2018

Which of the following mRNAs can be introduced into the cell to alter gene expression?

Think about which vitamin helps maintain the strength and structure of your skin, gums, and blood vessels. When it’s missing, your body’s ability to heal and hold tissues together falls apart — which vitamin is crucial for that?

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Tags: 2016

What does vitamin C deficiency lead to?

Think about the anomaly of water: as it cools, there’s a temperature at which water stops contracting and begins to expand before freezing. Consider the role of hydrogen bonding in this process, and how it affects the arrangement of water molecules.

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Tags: 2021

At what temperature does the water have the highest density?

Think about where the first amino acid (methionine) is placed to start the polypeptide chain — it needs to be positioned where peptide bonds will start forming. Which site holds the growing chain?

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Tags: 2016

During translation, the initiation codon of mRNA attaches to the ribosome at which site?

Hydrogen peroxide is a major source of oxidative stress in cells. What is the most reactive radical known to cause DNA and protein damage in oxidative stress conditions?

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Tags: 2019

Reduction and breakdown of hydrogen peroxide (H₂O₂) results in the production of which radical?

Imagine building a Lego structure out of sugar bricks. What kind of “connector” would you use to join the bricks together? It wouldn’t be the same connector you’d use for metal or plastic, would it?

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Tags: 2022

Which of the following bonds exists between the two monomers of a disaccharide?

Think about the molecule that blurs the line between a genetic material and a catalyst. Its discovery turned molecular biology upside down. What could it be?

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Tags: 2022

Which of the following types of RNA exhibits catalytic property?

Think about the type of glucose that forms the backbone of energy-storage polysaccharides in animals and plants. Consider the orientation of the hydroxyl group on the first carbon of the glucose molecule.

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Tags: 2020

Glycogen and starch are polymers of which of the following monosaccharides?

Which pyrimidine base, when exposed to UV light, tends to form abnormal bonds with its neighboring base, leading to DNA distortion and a high risk of skin cancer?”

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Tags: 2016

In xeroderma pigmentosum, there is accumulation of which substance due to ultraviolet light exposure?

Focus on the key feature of the semi-conservative model!

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Tags: 2022

Which of the following correctly describes the semi-conservative model of DNA replication?

Think of multiple ribosomes “reading” one book (mRNA) at the same time to produce many copies of a story (protein).

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Tags: 2018

Multiple ribosomes can bind to one mRNA strand during active protein synthesis. This structure is called which of the following?

 

Think about the functional diversity of the membrane — transportsignalingrecognition — and which component carries out most of these roles.

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Tags: 2019

Which of the following is the most abundant component of the cell membrane?

Granules on the ER are actually ribosomes—what do ribosomes do?

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Tags: 2021

What is the function of the endoplasmic reticulum with granules?

Consider a class of lipids that includes hormones like testosterone and estrogen, which are derived from a precursor molecule found in your body. What are these molecules called?

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Tags: 2017

Which one of these is a derived lipid?

“Which factor of RNA polymerase helps locate the promoter and initiate transcription in prokaryotes?”

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Tags: 2021

In prokaryotes, initiation of the transcription involves recognition of the initial site by which factor of RNA polymerase enzyme?

“Think of an enzyme with a safety lock—it needs a certain change to work.”

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Tags: 2019

What is a proenzyme?

Which field explains how ATP fuels life?

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Tags: 2019

What is the study of interconversion and utilization of energy in a living system called?

Cells must maintain a balance between membrane flexibility and rigidity to function properly. Which structure in the cell is directly responsible for interacting with the external environment and requires constant adjustment of its fluidity?

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Tags: 2019

Cholesterol is an important constituent of which of the following structures?

Consider the structures of amylopectin and amylose.

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Tags: 2018

Amylopectin and amylose are different structures within starch. Which of the following correctly differentiates between the two?

This strong glycosidic bond makes up the main component of plant cell walls, and humans lack the enzyme to break it down.

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Tags: 2023

Which type of glycosidic linkage is present in cellulose that cannot be digested with amylase present in saliva and pancreatic juice?

Think about the proportionality between strength of an acid with it’s pKa. Their relation to one another?

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Tags: 2021

Which of the following is true for strong acids?

“What type of bond between water molecules makes a drop of water keep its shape and resist being easily broken apart?”

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Tags: 2016

The properties of surface tension, high viscosity, and vaporization in water are caused by which of the following molecular interactions?

“Think about the classic lab test you perform to detect carbohydrates—specifically polysaccharides like starch. What dramatic color change occurs when iodine interacts with the coiled structure of amylose?”

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Tags: 2017

What is the color of starch in a positive iodine test

“Every amino acid shares the same amino group, carboxyl group, hydrogen, and central carbon—yet they behave so differently. What part of the molecule changes from one amino acid to another, giving each its unique identity and properties?”

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Tags: 2017

What do the characteristics of amino acids mainly depend on?

Think about why doctors screen for certain substances in urine and which metabolic imbalances would lead to certain substances appearing in urine. Which test would help quickly detect such abnormalities?

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Tags: 2018

Which of the following substances is the uristix test positive for?

Think about DNA replication and where new nucleotides attach — it’s always at the 3′ end because of the free hydroxyl group (-OH).

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Tags: 2019

Which of the following is true regarding the 5′ and 3′ end of DNA?

🔍 This mineral is essential for lysyl oxidase, an enzyme required for collagen cross-linking. A defect in its transport causes an X-linked disorder characterized by kinky hair and connective tissue defects.

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Tags: 2021

The deficiency of which of the following minerals is seen in Menkes disease, an X-linked recessive disorder, involving the defective cross-linking of collagen and elastic fibers?

Think about which enzyme actually breaks down lactose into simple sugars — that’s the key function of the lac Z gene product.

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Tags: 2018

In the lac operon, lac Z codes for which of the following?

Think of a self-annealing RNA structure that signals the end of transcription without additional proteins

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Tags: 2019

Which of the following is responsible for rho-independent termination of DNA transcription in prokaryotes?

Think of the amino acid that acts as both a methyl group donor and a starter for protein synthesis — and contains sulfur!

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Tags: 2019

Which of the following is a sulfur-containing amino acid?

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